- Filamentous fungi (FF) with inactivated mismatch repair
Title of Technology: Filamentous fungi (FF) with inactivated mismatch repair
Abstract: Filamentous fungal cells inactivated in the mismatch repair sys-tem, either permanently or transitorily. The technology also com-prises methods for preparing libraries in such FF, using cells inac-tivated in the mismatch repair system and growing cells for a pe-riod of time allowing duplication of genes.
Detailed Description: An active mismatch repair system as it is found in living organ-isms, minimizes the level of mutations and genetic variations, by eliminating differences between copies of the "same" gene in a cell. The current technology provides filamentous fungal cells with permanently or transitorily inactivated mismatch repair systems. It enables generation of levels of diversity not achievable by alternative methods. The technology can be particularly useful for generation of large libraries of variants of genes of interest, either directly in vivo in the FF cell or in vitro with subsequent transfor-mation into the FF cells of the invention.
Patent References
| Patent Number: |
Title of Patent: |
| WO 00/50567 |
Fungal cells with inactivated DNA mismatch repair systems PCT application |
Title of Technology: Gene library in filamentous fungi
Abstract: Gene library generation in filamentous fungi. The method includes the use of a fungal replication initiation origin.
Detailed Description: The method is the only known method enabling construction of gene libraries of any useful size in filamentous fungi relevant for production purposes. The fungal replication initiation origin pro-vides the high transformation frequency required for large library construction. Furthermore, it provides consistent copy number and expression levels in transformants, thus enabling direct qualitative and quantitiative assessment of members of the gene library.
Alternative methods of library construction typically involve the use of non-fungal hosts. For any product to be later produced in a filamentous fungus, experience shows little or unpredictable cor-relation in production potential between the organism used for li-brary construction and the ultimate production host when such al-ternative methods are applied. The current method therefore can be used to significantly increase efficiency in the discovery stage of a project by eliminating product leads or candidates with insuf-ficient expression potential.
Patent References
| Patent Number: |
Title of Patent: |
| WO 00/24883 |
Constructing and screening a DNA library of interest in filamentous fungal cells PCT application |