S. Bhargava; K. Wenger; M. R. Marten.
"Pulsed feeding during fed-batch Aspergillus oryzae fermentation leads to improved oxygen mass transfer."
Biotechnololy Prog. 19, 1091-1094 (2003)
Abstract
Productivity in many fungal fermentations is detrimentally affected by high broth viscosity and consequent reduced oxygen mass transfer capacity. The goal here was to determine whether pulsed feeding of limiting carbon in a fungal fermentation could lead to reduced viscosity and improved oxygen mass transfer. Compared to continuous feeding, pulsed addition of substrate led to smaller fungal elements, which resulted in a significant reduction in broth viscosity. This in turn led to higher dissolved oxygen concentrations and increased oxygen uptake rates during pulsed feeding.