Novozym 435 is probably the most commonly used lipase in biocatalysis. It is a highly versatile catalyst with activity towards a great variety of different substrates. The immobilized enzyme is a very thermostable and robust catalyst with activity in a number of organic solvents. It can be used both in batch and column reaction operations but is particularly well suited for use in fixed-bed reactors.
Even though Novozym 435 is used in industrial processes as a catalyst in the synthesis of simple esters (including polyesters) and amides and in the regioselective synthesis of carbohydrate monoesters, it is primarily used as a highly enantioselective catalyst in the synthesis of optically active alcohols, amines and carboxylic acids.