Enzymes for biocatalysis
Novozymes offers a broad portfolio of enzymes for biocatalysis. Find out which benefits this may give you.
In biocatalysis, natural catalysts - such as enzymes – are used in place of chemical catalysts in synthetic processes, opening more cost-effective and sustainable routes for the production of intermediates and active pharmaceutical ingredients (APIs).
- Reduction in raw material input
- Avoids use of costly chiral resolving agents or costly metal-based catalysts
- Lower equipment, labor and energy costs
- Very broad substrate specificity
- Shortened synthesis routes
- More batches resulting in increased capacity
- Avoids laborious protection and de-protection
- Higher yields
- All reactions can be performed with high regio- and enantioselectivity under mild reaction conditions
Improved quality of API/intermediate
- Fewer or no by-products, leading to reduced impurities in the final products
- High stereo-, regio-, and chemo-selectivity
- Less residual solvent carry over from reduced solvent use
- Reduction of waste products produced and solvent usage
- Higher energy savings
Lipases and immobilized lipases
Lipases (EC 18.104.22.168) are one of the most commonly used classes of enzymes in biocatalysis. They catalyze the hydrolysis of triacylglycerols to diacylglycerol, monoacylglycerol, glycerol and free fatty acids. The reaction reverses under anhydrous conditions and the enzyme is able to synthesize new molecules by esterification, alcoholysis and transesterification.
Regioselective hydrolysis of a triacylglycerol
Immobilization of lipase enzymes on a solid carrier has been shown to increase enzyme stability in non-aqueous solvents. Immobilization onto a solid carrier also facilitates recovery of products, recycling and reuse of catalyst and thereby decreases the cost of enzyme use in chemical processes.
Proteases (EC 22.214.171.124) are enzymes which conduct proteolysis by hydrolysis of the peptide bonds that link amino acids together in the polypeptide chain forming the protein. Proteases belong to the class of enzymes known as hydrolases catalyzing the reaction of hydrolysis of various bonds with the participation of a water molecule.
Proteolysis of a peptide bond