Immobilized lipase enzymes

Novozymes offers a portfolio of three immobilized lipase enzymes. Immobilization of lipase enzymes on a solid carrier leads to a number of benefits for biocatalysis.

Key applications of immobilized lipase enzymes

  • Enantioselective resolution of esters and amides
  • Acylation of alcohols and amines to form ester and amides
  • Mild hydrolysis or acylation of sensitive substrates
  • Kinetic resolution by transesterification of racemic alcohols
  • Kinetic resolution by hydrolysis of racemic ester
  • Desymmetrization of diesters

Key benefits of immobilized lipase enzymes

  • Better performance in non-aqueous solvents
  • Efficient recovery and separation of reaction product
  • Can be recycled for cost savings
  • Minimizes protein contamination of product
  • Enhanced stability from heat, organic solvents or autolysis
  • Higher catalyst productivity for cost-efficiency
  • Convenient and safer handling

Novozymes offers a product portfolio of three immobilized lipase enzymes:

Product name Applications Substrate specificity
Novozym® 435 Dynamic kinetic resolution of alcohols coupled with ruthenium catalyst. Dynamic kinetic resolution of amine coupled with metal racemizing agent Esters and alcohols
Lipozyme® TL IM Interesterification Esters
Novozym® 40086 Stereoselective transesterification and ester hydrolysis. Esters
Product name Source Optimum conditions
Novozym® 435 Candida antartica B pH 5-9
Temp 30-60°C
Lipozyme® TL IM Thermomyces lanuginosa pH 6-8
Temp 50-75°C
Novozym® 40086 Rhizomucour miehei pH 7-10
Temp 30-50°C