Lipase enzymes

Novozymes offers a portfolio of six lipases in liquid formulation. Lipases are one of the most commonly used classes of enzymes in biocatalysis.

Key applications of lipase enzymes

  • Enantioselective resolution of esters and amides
  • Acylation of alcohols and amines to form ester and amides
  • Mild hydrolysis or acylation of sensitive substrates
  • Kinetic resolution by transesterification of racemic alcohols
  • Kinetic resolution by hydrolysis of racemic ester
  • Desymmetrization of diesters

Novozymes offers six lipase enzymes in liquid formulation:

Product name Applications Substrate specificity
Lipozyme® CALB Resolution of racemic alcohols, amines, and acids. Preparation of optically active compounds from meso substrates. Selective acylation of different carbohydrates Esters and alcohols
Novocor® AD L Esterification of sterically hindered substrates Sterically hindered esters
Lipozyme® TL 100 L Transesterification, interesterification, ester hydrolysis and desymmetrization of esters

Commercial manufacture of Pregabalin
Esters and dicarboxylates
Resinase® HT Ester hydrolysis of triglycerides Esters and triglycerides
Palatase® 20000 L Hydrolysis of small fatty acids on the carboxylic acid part Esters
Novozym® 51032 Hydrolysis of triglycerides and a variety of molecules containing ester linkages. Specific hydrolytic activity on polyvinyl acetate Esters
Product name Source Optimum conditions
Lipozyme® CALB Candida antartica B pH 5-9
Temp 30-60°C
Novocor® AD L Candida antartica A pH 5-9
Temp 30-60°C
Lipozyme® TL 100 L Thermomyces lanuginosa pH 7-10
Temp 20-50°C
Resinase® HT Aspergillus oryzae pH 5-8
Temp up to 90°C
Palatase® 20000 L Rhizomucour miehei pH 7-10
Temp 30-50°C
Novozym® 51032 Humicola insolens pH 7-10
Temp 35-70°C
Product name Applications
Lipozyme® CALB Resolution of racemic alcohols, amines, and acids. Preparation of optically active compounds from meso substrates. Selective acylation of different carbohydrates
Novocor® AD L Esterification of sterically hindered substrates
Lipozyme® TL 100 L Transesterification, interesterification, ester hydrolysis and desymmetrization of esters

Commercial manufacture of Pregabalin
Resinase® HT Ester hydrolysis of triglycerides
Palatase® 20000 L Hydrolysis of small fatty acids on the carboxylic acid part
Novozym® 51032 Hydrolysis of triglycerides and a variety of molecules containing ester linkages. Specific hydrolytic activity on polyvinyl acetate