Protease enzymes

Novozymes offers a portfolio of seven protease enzymes for biocatalysis. Protease enzymes catalyze the reaction of hydrolysis of various bonds with the participation of a water molecule.

Key applications of protease enzymes

  • Kinetic resolution of amino acids by hydrolysis of racemic amino esters which can be converted into dynamic kinetic
  • resolution by addition of catalytic aldehyde1
  • Hydrolysis of selective amides2
  • Formation of low molecular weight peptides3
  • Transesterifications

Novozymes offers seven protease enzymes:

Product name Applications Substrate specificity
Alcalase® 2.4 L FG
(EC 3.4.21.62)
Stereoselective hydrolysis of amino esters
and selective esters;
suitable for hydrolysis of proteins;
used in transesterification and transpeptidation.
Serine endopeptidase
consisting primarily
of subtilisin A
Alcalase® 2.4 L FG
(EC 3.4.21.62)
Stereoselective hydrolysis of amino esters
and selective esters;
suitable for hydrolysis of proteins;
used in transesterification and transpeptidation.
Serine endopeptidase
consisting primarily
of subtilisin A
Savinase® 12 T
(EC 3.4.21.62)
Stereoselective hydrolysis of amino esters
and selective esters;
suitable for hydrolysis of proteins;
used in transesterification and transpeptidation.
Serine endopeptidase
consisting primarily
of subtilisin A
Savinase® 16 L
(EC 3.4.21.62)
Stereoselective hydrolysis of amino esters
and selective esters;
suitable for hydrolysis of proteins;
used in transesterification and transpeptidation.
Serine endopeptidase
consisting primarily
of subtilisin A
Esperase® 8.0 L
(EC 3.4.21.62)
Hydrolysis of internal
peptide bonds;
characterized by excellent performance
at elevated temperature
and pH.
Serine endopeptidase
consisting primarily
of subtilisin A
rTrypsin
(EC 3.4.21.4)
Hydrolysis of amide and
ester bonds of lysine
and arginine at carboxyl
terminal
Serine protease
Neutrase® 0.8 L
(EC 3.4.22)
Kinetic resolution of amino esters Metalloprotease
Product name Optimal conditions Unit activity* Format
EC number
Alcalase® 2.4 L FG
pH 7-9
Temp 30-65°C
2.4 AU-A/g liquid
(EC 3.4.21.62)
Alcalase® 2.5 L
pH 7-10
Temp 30-65°C
2.5 AU-A/g liquid
(EC 3.4.21.62)
Savinase® 12 T
pH 8-10
Temp 30-70°C
12 KNPU-S/g granulate
(EC 3.4.21.62)
Savinase® 16 L
pH 8-10
Temp 30-70°C
16 KNPU-S/g granulate
(EC 3.4.21.62)
Esperase® 8.0 L
pH 8-12.5 8 KNPU-E/g liquid
rTrypsin
(EC 3.4.21.4)
pH 7.8-8.0 800 USP/mg granulate
Neutrase® 0.8 L
(EC 3.4.22)
pH 7
Temp 40-50°C
0.8 AU/gotease liquid

* K = Kilo, AU = Anson Unit, NPU = Novo Protease Unit, 1 AU = 1NPU, ASNU = Asparaginace Unit, USP = Trypsin activity unit using USP Crystallised Trypsin Reference Standard. The activity is determined relative to a protease A standard. The result is given in the same units as the standard. 1 ASNU is the amount of enzyme that produces 1 μmol Ammonia per minute under the standard reaction conditions.

References:

1. D. A. Schichl, S. Enthaler, W. Holla, T. Riermeier, U. Kragl and M. Beller Eur. J. Org. Chem. 2008, 3506–3512
2. Mahmoud Mahmoudian, Andrew Lowdon, Martin Jones, Micheal Dawson, Christopher Wallis Tetrahedron Asymmetry 10 (1999) 1201-1206
3. Hou, R.-Z, Yang, Y. Li, G., Huang, Y.B., Wang, H., Zhang, N., Liu, Y. J.,
Li, X. and Zhang, X.Z., Biotechnol.Appl.Biochem., 2006, 44, 73