rTrypsin

Novozymes rTrypsin is a recombinant product manufactured by microbial fermentation. Unlike Trypsin, rTrypsin is animal origin-free.

Key applications of rTrypsin

  • Cell dissociation of cell lines including stem cells
  • Vaccine production and processing
  • Dissociation of dissected cells prior to cell fixing and sorting
  • Cleavage of proinsulin to yield intact insulin

Key benefits of rTrypsin

  • Produced by a recombinant manufacturing process
  • Not contaminated with chymotrypsin or carboxypeptidases
  • Animal origin-free manufacturing process eliminates the viral and animal-related contaminants
  • Allows simple replacement of bovine or porcine trypsin
  • Stability is not affected by Ca2+
  • Easy and convenient storage and routine handling
  • Consistent supply

Key characteristics of rTrypsin:

Product name
EC number
Application and substrates Unit activity and optimal conditions
Format
rTrypsin
(EC 3.4.21.4)
Hydrolysis of amide and ester bonds of lysine and arginine at carboxyl terminal 2.4 USP/mg
pH 7.8-8.0
Temp 30-65°C
granulate
USP = Trypsin activity unit using USP Crystallized Trypsin Reference Standard. The activity is determined relative to a protease A standard.