Novozymes today announces the launch of Spirizyme® 2.0 T and Spirizyme Ultra T for the European ethanol market. The two products are part of the Spirizyme T Portfolio, an advanced suite of glucoamylase enzymes with trehalase that deliver yield enhancing activities documented to provide the highest total sugar conversion in the industry.
“Reducing residual sugar, such as trehalose, through better conversion, generates up to EUR 850,000 more ethanol revenue for the plant,” says Thomas Schrøder, Vice President, Biorefining Commercial. “Extensive plant trials of the Spirizyme T products have shown that they reduce the amount of residual DP2 sugars by up to 70 percent.”
Trehalose, a type of sugar that is normally left unfermented in a standard ethanol plant, is targeted by the trehalase enzyme to produce glucose, which is then fermented to ethanol (see box).
Trehalose makes up a significant part of the ‘DP2 peak’, which is a measure of disaccharides and contributes to the overall residual sugar in an ethanol plant. The more DP2 an ethanol plant can convert, the more ethanol it will produce.
HPLC spectra are used to determine the concentration of different components within a fermentation sample. The two spectra here highlight that after trehalase is added to the fermentations, via the Spirizyme T portfolio of products, the resulting DP2 peak, including trehalose, is significantly reduced. The other components in the sample, represented by the various other peaks, remain unchanged.
Proven technology coupled with advanced technical services
|What is DP2?|
Ethanol is produced by the fermentation of sugar by yeast. Commercial production of fuel ethanol involves breakdown of starch in corn, wheat, and other feedstocks into simple sugars, fermentation of these sugars by yeast, and finally, recovery of the ethanol and byproducts such as animal feed.
Unfermented sugars go to waste, which is why ethanol producers are interested in technologies that increase efficiency. After fermentation, ethanol plant managers will run High-Performance Liquid Chromatography (HPLC) tests to measure the amount of residual sugar. The test measures four types of sugars: DP1 (single sugar chains such as glucose), DP2 (two-sugar chains such as trehalose), DP3 (3-sugar chains) and DP4 (everything else).
Reducing these sugar “peaks” is key to maximize ethanol production. At a typical ethanol plant, approx. 70 percent of DP2 is unfermentable trehalose, so by converting trehalose to a fermentable sugar, you can increase yield considerably. That is what the enzyme trehalase does.